User:Chris Chastain

Professional Preparation:

Education: B.S. 1978 Botany (with High Distinction), Colorado State University, Fort Collins M.S. 1982 Agronomy, University of Illinois, Urbana-Champaign Ph.D. 1985 Plant Physiology,	University of Illinois, Urbana-Champaign

Postdoctoral: University of Nebraska-Lincoln		Plant Biochemistry			1985-1987 Texas A&M University			Molecular Biology			1987-1989 CSIRO, Canberra, Australia			Plant Molecular Biology		1989-1992

Appointments: 2003-present	Professor, Department of Biosciences, Minnesota State University-Moorhead 1999-2003 	Associate Professor, Department of Biosciences, Minnesota State University-Moorhead 1994-1999	Assistant Professor, Department of Biosciences, Minnesota State University-Moorhead

Publications: †Chastain CJ, Xu W, Parsley K, Sarath G, Hibberd JM, Chollet R (2008) The pyruvate, orthophosphate dikinase regulatory proteins of Arabidopsis possess a novel, unprecedented Ser/Thr protein kinase primary structure. Plant J 53: 854--63

Burnell JN, Chastain CJ (2006) Cloning and expression of maize-leaf pyruvate, Pi dikinase regulatory protein gene. Biochem Biophys Res Comm 345 (2): 675-80

†Chastain CJ, Heck JW, Colquhoun TA, Voge DG, Gu XY (2006) Posttranslational regulation of pyruvate, orthophosphate dikinase in developing rice (Oryza sativa) seeds. Planta 224: 924–934

†Chastain CJ, Chollet R. (2003) Regulation of Pyruvate,Orthophosphate Dikinase by ADP/Pi-Dependent Reversible Phosphorylation in C3 and C4 Plants. Plant Physiology and Biochemistry 41: 523-532

Wallert, MA, Dunkirk SG, Chastain CJ, Brisch E, Malott ML, Provost JJ (2002) Shifting the Cultural Paradigm of the Biology and Chemistry Departments at Minnesota State University Moorhead: Development of a Research-Centered Undergraduate Biotechnology Curriculum. The FASEB Journal, 16(4): A930

†Chastain CJ, Fries JP, Vogel JA, Randklev CL,  Vossen AP,  Dittmer SK,  Watkins EE,  Fiedler LJ,  Wacker SA, Meinhover KC, Sarath G, Chollet R (2002) Pyruvate,orthophosphate dikinase in leaves and chloroplasts of C3 plants undergoes light/dark-Induced reversible phosphorylation. Plant Physiol 128:1368-1378

†Chastain CJ, Botschner M, Harrington GS, Thompson BJ, Mills SJ, Sarath G, Chollet R (2000) Further analysis of maize C4-pyruvate,orthophosphate dikinase phosphorylation by its bifunctional regulatory protein, RP, using selective substitutions of the regulatory Thr-456 and catalytic His-458 residues. Arch Biochem Biophys 375:165-170

†Chastain CJ, Lee ME, Moorman MA, Shameekumar P, Chollet R (1997) Site-directed mutagenesis of maize recombinant C4-pyruvate,orthophosphate dikinase at the phosphorylatable target threonine residue. FEBS Lett 413:169-173